Recombinant Mouse Phosphoglycerate Mutase 1, His-tagged

• Cat.No.: Pgam1-1955M
• Product Overview: Recombinant mouse Pgam1protein,fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.

Specification

• Cat. No.: Pgam1-1955M
• Description: Pgam1 belongs to thephosphoglycerate mutase family. This protein is important components ofglucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes thereversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate(2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing,in different tissues, different proportions of a slow-migrating muscle (MM)isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB).
• Form: Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT.
• Molecular Weight: 31.4 kDa (278aa)confirmed by MALDI-TOF
• Purity: > 95% by SDS-PAGE
• Concentration: 1 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMAAYKL VLIRHGESAW NLENRFSGWY DADLSPAGHE EAKRGGQALRDAGYEFDICF TSVQKRAIRT LWTVLDAIDQ MWLPVVRTWR LNERHYGGLT GLNKAETAAK HGEAQVKIWRRSYDVPPPPM EPDHPFYSNI SKDRRYADLT EDQLPSCESL KDTIARALPF WNEEIVPQIK EGKRVLIAAHGNSLRGIVKH LEGLSEEAIM ELNLPTGIPI VYELDKNLKP IKPMQFLGDE ETVRKAMEAV AAQGKVKK
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• OfficialSymbol: Pgam1
• Pathway: Gluconeogenesis; Glucose metabolism; Glycolysis;Glycolysis / Gluconeogenesis; Glycolysis and Gluconeogenesis; Glycolysis,core module involving three-carbon compounds; Metabolic pathways; gluconeogenesis;glycolysis I; glycolysis II; glycolysis III

Gene Information

• Gene Name: Pgam1phosphoglycerate mutase 1 [ Mus musculus ]
• Synonyms: Pgam1; phosphoglyceratemutase 1; Pgam-1;2310050F24Rik; PGAM-B; EC 3.1.3.13; EC 5.4.2.1; EC 5.4.2.4; BPG-dependent PGAM1; Phosphoglycerate mutase isozymeB
• Gene ID: 18648
• mRNA Refseq: NM_023418
• Protein Refseq: NP_075907
• UniProt ID: Q9DBJ1
• Chromosome Location: 19