Recombinant Human PGAM1 293 Cell Lysate

Description :	Antigen standard for phosphoglycerate mutase 1 (brain) (PGAM1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source :	HEK 293 cells
Species :	Human
Components :	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size :	0.1 mg
Storage Instruction :	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications :	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag :	Non

Gene Name	PGAM1 phosphoglycerate mutase 1 (brain) [ Homo sapiens ]
Official Symbol	PGAM1
Synonyms	PGAM1; phosphoglycerate mutase 1 (brain); PGAMA; phosphoglycerate mutase 1; PGAM B; Phosphoglycerate mutase A; nonmuscle form; BPG-dependent PGAM 1; phosphoglycerate mutase isozyme B; phosphoglycerate mutase A, nonmuscle form; PGAM-B;
Gene ID	5223
mRNA Refseq	NM_002629
Protein Refseq	NP_002620
MIM	172250
UniProt ID	P18669
Chromosome Location	10q25.3
Pathway	Gluconeogenesis, organism-specific biosystem; Gluconeogenesis, oxaloacetate =>fructose-6P, organism-specific biosystem; Gluconeogenesis, oxaloacetate => fructose-6P, conserved biosystem; Glucose metabolism, organism-specific biosystem; Glycine, serine and threonine metabolism, organism-specific biosystem;
Function	bisphosphoglycerate 2-phosphatase activity; bisphosphoglycerate mutase activity; hydrolase activity; isomerase activity; phosphoglycerate mutase activity; phosphoglycerate mutase activity; protein kinase binding;

COA