Active Recombinant Rhesus FGFR1 protein, hFc-tagged

• Cat.No.: FGFR1-8817C
• Product Overview: Recombinant Rhesus FGFR1 protein(NP_001253576.1)(Met1-Tyr283), fused with hFc tag, was expressed in HEK293.

Specification

• Product Overview: Recombinant Rhesus FGFR1 protein(NP_001253576.1)(Met1-Tyr283), fused with hFc tag, was expressed in HEK293.
• Source: HEK293
• Species: Rhesus
• Tag: Fc
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Immobilized humen FGF10 (10573-HNAE) at 10 μg/ml (100 μl/well) can bind Rhesus FGFR1-Fc with a linear range of 31.25-2000 ng/mL.2. Measured by its ability to inhibit FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.1-0.6 ng/mL
• Molecular Mass: The recombinant rhesus FGFR1 comprises 503 amino acids and has a calculated molecular mass of 56.2 KDa.
• Protein length: Met1-Tyr283
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.