Active Recombinant Mouse Pgam1 protein, His-tagged (Bioactivity Validated)

• Cat.No.: PGAM1-14M
• Product Overview: Recombinant mouse PGAM1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Description: PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB).
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
• Bio-activity: Specific activity is >150units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37centigrade.
• Molecular Mass: 31.4 kDa (278aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAAYKL VLIRHGESAW NLENRFSGWY DADLSPAGHE EAKRGGQALR DAGYEFDICF TSVQKRAIRT LWTVLDAIDQ MWLPVVRTWR LNERHYGGLT GLNKAETAAK HGEAQVKIWR RSYDVPPPPM EPDHPFYSNI SKDRRYADLT EDQLPSCESL KDTIARALPF WNEEIVPQIK EGKRVLIAAH GNSLRGIVKH LEGLSEEAIM ELNLPTGIPI VYELDKNLKP IKPMQFLGDE ETVRKAMEAV AAQGKVKK
• Purity: > 95% by SDS - PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20centigrade or -70centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: Pgam1 phosphoglycerate mutase 1 [ Mus musculus ]
• Official Symbol: PGAM1
• Synonyms: PGAM1; phosphoglycerate mutase 1; PGAM-B; BPG-dependent PGAM 1; Pgam-1
• Gene ID: 18648
• mRNA Refseq: NM_023418
• Protein Refseq: NP_075907
• Pathway: Gluconeogenesis, organism-specific biosystem; Gluconeogenesis, oxaloacetate => fructose-6P, organism-specific biosystem; Gluconeogenesis, oxaloacetate => fructose-6P, conserved biosystem; Glucose metabolism, organism-specific biosystem; Glycine, serine and threonine metabolism, organism-specific biosystem
• Function: phosphoglycerate mutase activity; phosphoglycerate mutase activity; protein kinase binding