Recombinant Mouse F2 protein, His-tagged

• Cat.No.: F2-7769M
• Product Overview: Recombinant Mouse F2 aa. (Thr325~Gly618 (Accession # P19221)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Thr325~Gly618
• Description: This gene encodes a vitamin K-dependent glycoprotein coagulation factor that plays an important role in the process of blood coagulation and hemostasis. The encoded protein is an inactive zymogen that undergoes enzymatic cleavage by the coagulation factor Xa to form an active serine protease that converts soluble fibrinogen to insoluble fibrin clot. Most of the mice lacking the encoded protein die at an embryonic stage due to defects in yolk sac vasculature, while the rare nenonates succumb to hemorrhage on the first postnatal day.
• Form: Freeze-dried powder
• Molecular Mass: 35.5 kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.1.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
• Reconstitution: Reconstitute in PBS, PH7.2-PH7.4.

Gene Information

• Gene Name: F2 coagulation factor II [ Mus musculus (house mouse) ]
• Official Symbol: F2
• Synonyms: Cf2; FII; Cf-2; prothrombin; thrombin
• Gene ID: 14061
• mRNA Refseq: NM_010168.3
• Protein Refseq: NP_034298.1
• UniProt ID: P19221