Native Pig F2

• Cat.No.: F2-647P
• Product Overview: Thrombin is from Procine blood.

Specification

• Description: Thrombin is a "trypsin-like" serine protease protein. Prothrombin (coagulation factor II) is proteolytically cleaved to form thrombin in the coagulation cascade, which ultimately results in the stemming of blood loss. As a result of cleaving, thrombin has two chains: a light chain (A chain, MW ~6 kDA) and a heavy chain (B chain, MW ~31 kDa). These two chains are joined by one disulfide bond. Functions of thrombin are that it converts soluble fibrinogen into insoluble strands of fibrin, as well as catalyzes many other coagulation-related reactions. Thrombin also contains g-carboxyglutamyl residues. These modified glutamyl residues are the result of carboxylation by a microsomal enzyme, vitamin Kdependent carboxylase. g-Carboxyglutamyl residues are necessary for the Ca2+-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
• Source: Procine Blood
• Species: Procine
• Form: Lyophilized Powder from 20 mM PBS, pH 7.4 containing 0.9% NaCl.
• Purity: >95% by SDS-PAGE and HPLC analyses.
• Optimum pH: Stable at neutral pH
• Notes: 1. Avoid keeping with heavy metal; 2. Recommended to aliquot the solutions in plastic tubes.Optimal Cleavage Sites1. A-B-Pro-Arg-||-X-Y where A and B are hydrophobic amino acids and X and Y are nonacidic amino acids 2. Gly-Arg-||-Gly
• Storage: Two year when stored at -20 centigrade and stable at room temperature for 3 weeks. Avoid repeated freeze/thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile water to a concentration of 10-100 u/ml. Stock solutions should be apportioned into working aliquots and stored at -20°C.
• Tag: Non

Gene Information

• Gene Name: F2 coagulation factor II (thrombin) [ Sus scrofa ]
• Official Symbol: F2
• Synonyms: prothrombin; thrombin
• Gene ID: 100144442
• mRNA Refseq: NM_001122985
• Protein Refseq: NP_001116457
• UniProt ID: Q19AZ8
• Chromosome Location: 2; 2
• Pathway: Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Neuroactive ligand-receptor interaction, conserved biosystem
• Function: calcium ion binding; serine-type endopeptidase activity