Recombinant Human Coagulation Factor II (F2) protein, His-tagged

• Cat.No.: F2-305H
• Product Overview: Recombinant Human Coagulation Factor II (F2) protein (Gln25-Glu622), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 598
• Description: Prothrombin is also known as coagulation factor II (F2),which can be cleaved into the following 4 chains: activation peptide fragment 1, activation peptide fragment 2, thrombin light chain and thrombin heavy chain. Coagulation factor II (F2) is able to form a heterodimer with SERPINA5. The mutations of F2 gene can result in a very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. Furthermore, thrombin converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Coagulation factor II (F2) can also play an important role in blood homeostasis, inflammation and wound healing.
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 69.5 kDa. The protein migrates as 85-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >95% as determined by reduced SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: F2
• Official Symbol: F2
• Synonyms: F2; coagulation factor II (thrombin); prothrombin; serine protease; prothrombin B-chain; PT; THPH1
• Gene ID: 2147
• mRNA Refseq: NM_000506
• Protein Refseq: NP_000497
• MIM: 176930
• UniProt ID: P00734