Recombinant Porcine Thrombin

• Cat.No.: Thrombin-24P

Specification

• Cat. No.: Thrombin-24P
• Description: Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.
• Source: Porcine Blood
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized Thrombin in sterile 18 MΩ-cm H₂O.
• Formulation: Lyophilized Powder from 20 mM PBS, pH 7.4 containing 0.9% NaCl.
• Biological Activity: One unit is defined as the amount of enzyme needed to cleave 1 mg of fusion protein in 16 hours to 95% completion at 20°C in a buffer containing 25 mM Tris-HCl, pH 8.4, 150 mM NaCl, and 2.5 mM CaCl₂.
• Storage: Lyophilized Thrombin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IPF1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Tag: Non
• Synonyms: F2; PT; coagulation factor II (thrombin); coagulation factor II; serine protease; prothrombin B-chain; EC 3.4.21.5.

Gene Information

• Synonyms: F2; PT; coagulation factor II (thrombin); coagulation factor II; serine protease; prothrombin B-chain; EC 3.4.21.5.

Reviews

01/01/2022

Quality control is evident. This product ensures accuracy in our studies.

01/27/2019

Our lab swears by this protein. Fast shipping is a definite bonus.

12/18/2018

Consistently reliable. The shipping speed is appreciated in our fast-paced lab.

Q&As

Can you discuss the role of the F2 gene in the synthesis of vitamin K-dependent clotting factors, and how disruptions in this process may lead to coagulation disorders?

The F2 gene plays a crucial role in the synthesis of vitamin K-dependent clotting factors, including prothrombin. Disruptions in this process, often due to genetic or nutritional factors, can contribute to coagulation disorders, impacting hemostasis and blood clotting.

How is the F2 gene regulated at the transcriptional level, and what factors influence prothrombin synthesis in hepatocytes?

Transcriptional regulation of the F2 gene involves factors like vitamin K-dependent gamma-carboxylation. Hepatocytes synthesize prothrombin under the influence of vitamin K and other coagulation factors.

The F2 gene encodes prothrombin, a crucial factor in blood coagulation. Prothrombin is converted to thrombin, a key enzyme in the clotting cascade, facilitating fibrin formation.

The F2 gene encodes prothrombin, a crucial factor in blood coagulation. Prothrombin is converted to thrombin, a key enzyme in the clotting cascade, facilitating fibrin formation.

What are the implications of genetic variations in the F2 gene, particularly polymorphisms, on prothrombin function and susceptibility to thrombotic or bleeding disorders?

Genetic variations in F2, including polymorphisms, can influence prothrombin function. Certain variants may predispose individuals to thrombotic or bleeding disorders.

How does genetic variation in the F2 gene impact prothrombin levels, and what are the consequences for an individual's risk of thrombotic events?

Genetic variations in the F2 gene can influence prothrombin levels, affecting an individual's propensity for thrombotic events. Certain variants may predispose individuals to a higher or lower risk of abnormal blood clot formation.