Recombinant Mouse Col1a1 Protein, His&GST-tagged

• Cat.No.: Col1a1-816M
• Product Overview: Recombinant Mouse Col1a1 Protein (Gly22-Ser154) with His&GST tag was expressed in E. coli.

Specification

• Description: This gene encodes the alpha-1 subunit of the fibril-forming type I collagen, the most abundant protein of bone, skin and tendon extracellular matrices. The encoded protein, in association with alpha-2 subunit, forms heterotrimeric type I procollagen that undergoes proteolytic processing during fibril formation. Mice lacking the encoded protein die in utero caused by the rupture of a major blood vessel. Transgenic mice expressing significantly lower levels of this gene exhibit morphological and functional defects in mineralized and non-mineralized connective tissue and, progressive loss of hearing.
• Source: E. coli
• Species: Mouse
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 43.7 kDa; Accurate Molecular Mass: 49 kDa
• Protein length: Gly22-Ser154
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Col1a1 collagen, type I, alpha 1 [ Mus musculus (house mouse) ]
• Official Symbol: Col1a1
• Synonyms: COL1A1; collagen, type I, alpha 1; collagen alpha-1(I) chain; alpha-1 type 1 collagen; alpha-1 type I collagen; procollagen, type I, alpha 1; Cola1; Mov13; Cola-1; Mov-13; Col1a-1
• Gene ID: 12842
• mRNA Refseq: NM_007742
• Protein Refseq: NP_031768
• UniProt ID: P11087

Reviews

10/18/2022

Timely delivery, so I got the desired experimental results in the shortest possible time.

08/13/2022

Very good after-sales service, an excellent shopping experience!

03/15/2022

The product brochure is very detailed and has comprehensive basic information about the COL1A1 protein.

Q&As

What diseases is COL1A1 involved in?

COL1A1 has been associated with the study of colorectal, gastric and pancreatic cancers.

What are the effects of inhibiting COL1A1 expression?

Inhibition of COL1A1 expression can affect the expression of various genes, thereby inhibiting tumor cell proliferation and promoting cell apoptosis.

Where is COL1A1 mainly expressed?

COL1A1 is highly expressed in heart and muscle tissues.

What are the components of COL1A1?

COL1A1 consists of three conserved structural domains, Collagen, COLFI and VWC.

What are the biological functions of COL1A1?

COL1A1 is involved in maintaining tissue structure and angiogenesis.

How does COL1A1 expression change in colorectal cancer tissues?

COL1A1 expression is significantly upregulated in colorectal cancer tissues.