Active Recombinant Human collagen type I alpha 1 chain Protein, His tagged

• Cat.No.: COL1A1-114H
• Product Overview: Active Recombinant Human collagen type I alpha 1 chain Protein (Gln23-Lys277 + Gly1094-Leu1464) with an N-terminal 6-His tag was expressed in Chinese Hamster Ovary cell line.

Specification

• Description: This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
• Source: CHO
• Species: Human
• Tag: N-6×His
• Protein length: 23-277 aa and 1094-1464 aa
• Bio-activity: Measured by the cleavage of its C-terminal propeptide by Recombinant Human BMP-1/PCP. > 50 % of full-length Pro-Collagen I alpha 1 is cleaved by Recombinant Human BMP-1/PCP, as measured under the described conditions.
• Molecular Mass: 65 kDa (full-length), 40 kDa, 28 kDa
• N-terminal Sequence Analysis: His & Asp1219
• Endotoxin: < 1.0 EU/μg of the protein by the LAL method.
• Purity: > 80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Shipping: The product is shipped with dry ice or equivalent.
• References: 1. Canty, E.G. et al. (2005) J. Cell Sci. 118:1341.
  2. Han, S. et al. (2008) J. Mol. Biol. 383:122.

Gene Information

• Gene Name: COL1A1 collagen type I alpha 1 chain [ Homo sapiens (human) ]
• Official Symbol: COL1A1
• Synonyms: COL1A1; collagen type I alpha 1 chain; OI1; OI2; OI3; OI4; EDSC; CAFYD; EDSARTH1; collagen alpha-1(I) chain; alpha-1 type I collagen; alpha1(I) procollagen; collagen alpha 1 chain type I; collagen alpha-1(I) chain preproprotein; collagen of skin, tendon and bone, alpha-1 chain; collagen, type I, alpha 1; pro-alpha-1 collagen type 1; type I proalpha 1; type I procollagen alpha 1 chain
• Gene ID: 1277
• mRNA Refseq: NM_000088
• Protein Refseq: NP_000079
• MIM: 120150
• UniProt ID: P02452