Recombinant Human UNG 293 Cell Lysate

• Cat.No.: UNG-497HCL

Specification

• Description: Antigen standard for uracil-DNA glycosylase (UNG), nuclear gene encoding mitochondrial protein, transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: UNG uracil-DNA glycosylase [ Homo sapiens ]
• Official Symbol: UNG
• Synonyms: UNG; uracil-DNA glycosylase; DGU; HIGM4; UDG; UNG1; UNG2; uracil DNA glycosylase 1; uracil DNA glycosylase 2; uracil-DNA glycosylase 1, uracil-DNA glycosylase 2; UNG15; DKFZp781L1143
• Gene ID: 7374
• mRNA Refseq: NM_003362
• Protein Refseq: NP_003353
• MIM: 191525
• UniProt ID: P13051
• Chromosome Location: 12q23-q24.1
• Pathway: Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem; Primary immunodeficiency, organism-specific biosystem; Primary immunodeficiency, conserved biosystem

Reviews

09/22/2022

The technician guided me through the process, which led to satisfactory results.

07/14/2022

The UNG protein product received was as advertised, top quality and good value for money.

05/04/2022

Prompt delivery helped me get satisfactory experimental results in a short period of time.

Q&As

What biological processes are UNG involved in?

UNG is involved in DNA repair, base excision repair and apoptosis.

Where is UNG located?

UNG is localized in the nucleus, nucleoplasm, and mitochondria.

What protein does UNG shear to produce?

UNG can be sheared to produce UNG1 and UNG2.

Where is UNG primarily expressed?

UNG is mainly expressed in testis, thymus and skeletal muscle.