Recombinant E.coli uracil-DNA-glycosylase, His-tagged

• Cat.No.: ung-282E
• Product Overview: ung, 1-229aa, E.coli, His tag, E.coli

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Protein Length: 1-229 a.a.
• Description: Ung, also known as Uracil-DNA glycosylase, is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. After a mutation occurs, the mutagenic threat of uracil propagates through any subsequent DNA replication steps. Recombinant E.coli ung protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
• Molecular Mass: 28.1 kDa(252aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMANELTW HDVLAEEKQQ PYFLNTLQTV ASERQSGVTI YPPQKDVFNA FRFTELGDVK VVILGQDPYH GPGQAHGLAF SVRPGIAIPP SLLNMYKELE NTIPGFTRPN HGYLESWARQ GVLLLNTVLT VRAGQAHSHA SLGWETFTDK VISLINQHRE GVVFLLWGSH AQKKGAIIDK QRHHVLKAPH PSPLSAHRGF FGCNHFVLAN QWLEQRGETP IDWMPVLPAE SE
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ung?uracil-DNA-glycosylase [?Escherichia coli str. K-12 substr. MG1655?]
• Official Symbol: ung
• Synonyms: ung; ECK2578; JW2564; uracil-DNA-glycosylase; NP_417075.1; EC 3.2.2.-
• Gene ID: 947067
• Protein Refseq: NP_417075
• Pathway: Base excision repair