Recombinant Human HLF, His-tagged

• Cat.No.: HLF-3618H
• Product Overview: Recombinanthuman HLF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: HLF, also known as hepatic leukemia factor, belongs to the bZIP family and the PAR (proline and acidic-rich) subfamily of transcription regulatory proteins. HLF binds DNA specifically as a homodimer or heterodimer with other PAR factors. It is highly expressed in liver, with lower levels in lung and kidney and functions as a potent trans-activator.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.2M NaCl,0.1mM PMSF
• Molecular Weight: 35.3 kDa(315aa),confirmed by MALDI-TOF
• Purity: >85% by SDS - PAGE
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MEKMSRPLPL NPTFIPPPYG VLRSLLENPL KLPLHHEDAFSKDKDKEKKL DDESNSPTVP QSAFLGPTLWDKTLPYDGDT FQLEYMDLEE FLSENGIPPSPSQHDHSPHP PGLQPASSAA PSVMDLSSRA SAPLHPGIPS PNCMQSPIRP GQLLPANRNTPSPIDPDTIQ VPVGYEPDPA DLALSSIPGQ EMFDPRKRKF SEEELKPQPM IKKARKVFIPDDLKDDKYWA RRRKNNMAAK RSRDARRLKENQIAIRASFL EKENSALRQE VADLRKELGKCKNILAKYEA RHGPL
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: HLF hepatic leukemia factor [ Homo sapiens ]
• Official Symbol: HLF
• Synonyms: HLF; MGC33822; hepatic leukemia factor
• Gene ID: 3131
• mRNA Refseq: NM_002126
• Protein Refseq: NP_002117
• MIM: 142385
• UniProt ID: Q16534
• Chromosome Location: 17q22
• Function: DNA binding; double-stranded DNA binding; protein dimerization activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity