Active Recombinant Human INS, Met, His&Lys-tagged

• Cat.No.: INS-607H
• Product Overview: Recombinant Human INS (Accession # NP_000198) Phe25-Asn110, fused with an N-terminal Met, 6-His tag and Lys, was produced in E. coli-derived.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 25-110 a.a.
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.The ED50 for this effect is typically 0.15 - 0.75 µg/mL.
• Molecular Mass: Recombinant Human INS, Met, His&Lys-tagged has a calculated MW of 10.5 kDa. In SDS-PAGE migrates as 9 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: INS insulin [ Homo sapiens ]
• Official Symbol: INS
• Synonyms: INS; insulin; proinsulin; ILPR; IRDN; IDDM2; MODY10
• Gene ID: 3630
• mRNA Refseq: NM_000207
• Protein Refseq: NP_000198
• UniProt ID: P01308
• Chromosome Location: 11p15.5