Recombinant Human INS Protein (Phe25-Thr54, Gly90-Thr110), N-His tagged

• Cat.No.: INS-2368H
• Product Overview: Recombinant human INS (Phe25-Thr54, Gly90-Thr110) protein fused with the N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified, including insulin-dependent diabetes mellitus, permanent neonatal diabetes diabetes mellitus, maturity-onset diabetes of the young type 10 and hyperproinsulinemia. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: 11.8 kDa
• Protein length: Phe25-Thr54, Gly90-Thr110
• Purity: >90 % as determined by SDS-PAGE.
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in PBS or lyophilized from PBS.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: INS insulin [ Homo sapiens (human) ]
• Official Symbol: INS
• Synonyms: INS; insulin; proinsulin; ILPR; IRDN; IDDM2; MODY10
• Gene ID: 3630
• mRNA Refseq: NM_000207
• Protein Refseq: NP_000198
• MIM: 176730
• UniProt ID: P01308