Recombinant Human Insulin

• Cat.No.: INS-5308H
• Product Overview: Recombinant HumanInsulin is a two chain, non-glycosylated polypeptide chain containing 51amino acids and has a MW = 5.8 kDa.

Specification

• Cat. No.: INS-5308H
• Description: INS is a hormonecentral to regulating carbohydrate and fat metabolism in the body. INS causescells in the liver, muscle, and fat tissue to take up glucose from the blood,storing it as glycogen in the liver and muscle. INS stops the use of fat asan energy source by inhibiting the release of glucagon. With the exception ofthe metabolic disorder diabetes mellitus and Metabolic syndrome, INS isprovided within the body in a constant proportion to remove excess glucosefrom the blood, which otherwise would be toxic.
• Source: E. coli
• Species: Human
• Form: Lyophilized from asterile, concentrated (1 mg/ml) solution with no additives.
• Molecular Weight: 5.8 kDa
• Purity: >98.0% asdetermined by RP-HPLC and SDS-PAGE.
• Endotoxin Level: <0.1 ng/µg ofrecombinant human Insulin
• Biological Activity: 28 units/mg
• Reconstitution: Centrifuge vialprior to opening. Add sterile 0.005 N HCL to the vial to fully solubilize theprotein to a concentration not more than 1 mg/ml.
• Storage: Lyophilized productis stable at room temperature for 3 weeks, but it is recommended to store thelyophilized product desiccated at -20°C to -80°C. Upon reconstitution,protein should be stored at 2-4°C for one week and for future use at -20°C to-80°C. Add a carrier protein (0.1% HSA or BSA) as a stabilizer for long termstorage. Please note that the addition of any carrier protein into thisproduct may produce unwanted endotoxin. This depends upon the particularapplication employed. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: INS
• Tag: Non

Gene Information

• Gene Name: INS insulin [ Homo sapiens]
• Synonyms: INS; insulin; ILPR;IRDN; IDDM2; MODY10; proinsulin; OTTHUMP00000011161; OTTHUMP00000011162;OTTHUMP00000196036; OTTHUMP00000196038; OTTHUMP00000217519
• Gene ID: 3630
• mRNA Refseq: NM_000207
• Protein Refseq: NP_000198
• MIM: 176730
• UniProt ID: P01308
• Chromosome Location: 11p15.5
• Pathway: ATF-2 transcriptionfactor network; Adipogenesis; Arf6 trafficking events; Diabetes pathways;FOXA2 and FOXA3 transcription factor networks; Folate Metabolism; IRS activation;Insulin receptor recycling; Insulin signaling pathway; Maturity onsetdiabetes of the young; Progesterone-mediated oocyte maturation; Regulation ofInsulin Secretion; SHC-related events; Type I diabetes mellitus; mTORsignaling pathway
• Function: hormone activity;insulin receptor binding; insulin-like growth factor receptor binding;protein binding