Active Recombinant Human IGF2 Protein, Fc & Avi-tagged, Biotinylated

• Cat.No.: IGF2-215H
• Product Overview: Biotinylated Human IGF-II, Fc Tag (IG2-H82F9) is expressed from human HEK293 cells. It contains AA Ala 25 - Glu 91 (Accession # P01344).

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&Avi
• ProteinLength: 25-91 a.a.
• Form: Lyophilized from sterile 50 mM tris, 100 mM glycine, pH7.5, 10% Trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA against immobilized Human IGFBP-3, His Tag (0.5 μg/well). The linear range is 0.008-0.1 μg/mL.
• Molecular Mass: This protein carries an Avi tag at the N-terminus, followed by a human IgG1 Fc fragment. The protein has a calculated MW of 35.4 kDa. The protein migrates as 40 kDa on a SDS-PAGE gel under reducing condition and 66 kDa under non-reducing condition.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: IGF2 insulin-like growth factor 2 (somatomedin A) [ Homo sapiens ]
• Official Symbol: IGF2
• Synonyms: IGF2; insulin-like growth factor 2 (somatomedin A); C11orf43, chromosome 11 open reading frame 43; insulin-like growth factor II; FLJ44734; somatomedin-A; insulin-like growth factor type 2; IGF-II; PP9974; C11orf43; FLJ22066
• Gene ID: 3481
• mRNA Refseq: NM_000612
• Protein Refseq: NP_000603
• UniProt ID: P01344
• Chromosome Location: 11p15.5
• Pathway: Apoptosis, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Endochondral Ossification, organism-specific biosystem; Hedgehog Signaling Pathway, organism-specific biosystem; Regulation of Insulin-like Growth Factor (IGF) Activity by Insulin-like Growth Factor Binding Proteins (IGFBPs), organism-specific biosystem
• Function: growth factor activity; hormone activity; insulin receptor binding; insulin-like growth factor receptor binding; protein binding; protein serine/threonine kinase activator activity; receptor activator activity