Recombinant Rat Mdh1 Protein, His&GST-tagged

• Cat.No.: Mdh1-1770R
• Product Overview: Recombinant Rat Mdh1 Protein (Ser2-Ala334) with N-His&GST tag was expressed in E. coli.

Specification

• Species: Rat
• Source: E.coli
• Tag: GST&His
• Protein Length: Ser2-Ala334
• Description: This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 66.3 kDa
  Accurate Molecular Mass: 66 kDa
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Mdh1 malate dehydrogenase 1, NAD (soluble) [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Mdh1
• Synonyms: MDH1; malate dehydrogenase 1, NAD (soluble); malate dehydrogenase, cytoplasmic; malate dehydrogenase, soluble; cytosolic malate dehydrogenase; Malate dehydrogenase-like enzyme; MDL1; Mdhl; Mor2
• Gene ID: 24551
• mRNA Refseq: NM_033235
• Protein Refseq: NP_150238
• UniProt ID: O88989