Recombinant Rat GDF15 protein, His-tagged

• Cat.No.: Gdf15-26R
• Product Overview: Recombinant Rat GDF15 protein(Q9Z0J6)(Ser189-Ala303), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Source: E.coli
• Species: Rat
• Tag: N-His
• Protein length: Ser189-Ala303
• Form: Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Rat GDF-15, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 13.68 kDa. The protein migrates to 15-17 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE.
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).

Gene Information

• Gene Name: Gdf15 growth differentiation factor 15 [ Rattus norvegicus ]
• Official Symbol: GDF15
• Synonyms: GDF15; growth differentiation factor 15; growth/differentiation factor 15; GDF-15
• Gene ID: 29455
• mRNA Refseq: NM_019216
• Protein Refseq: NP_062089