Active Native Human GDF15 Protein

• Cat.No.: GDF15-27680TH
• Product Overview: Native Human GDF15 Protein was purified from Cell Culture.

Specification

• Species: Human
• Tag: Non
• Description: GDF-15 belongs to the TGF-β cytokine family whose members play an important role during prenatal development and postnatal growth, remodeling and maintenance of a variety of tissues and organs. GDF-15 is expressed predominantly in placenta and to a much lesser extent in various other tissues. The presence of GDF-15 in amniotic fluid and its elevated levels in the sera of pregnant women suggest a role for GDF-15 in gestation and embryonic development. GDF-15 generally exerts tumor suppressive activities and is one of the predominant factors produced and secreted in response to activation of the p53 pathway. Interestingly, the serum level of GDF-15 is positively correlated with neoplastic progression of several tumor types, including certain colorectal, pancreatic, and prostate cancers. Human GDF-15/MIC-1 is a disulfide linked homodimeric protein consisting of two 114 amino-acid polypeptide chains.
• Form: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM sodium citrate, pH 3.0.
• Bio-activity: Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3/E1 osetoblast cells. The expected ED50 for this effect is 1.0-3.0 μg/ml.
• AA Sequence: ARNGDHCPL GPGRCCRLH TVRASLEDL GWADWVLSP REVQVTMCI GACPSQFRA ANMHAQIKT SLHRLKPDT VPAPCCVPA SYNPMVLIQ KTDTGVSLQ TYDDLLAKD CHCI
• Endotoxin: < 1EU per 1µg (determined by the LAL method)
• Purity: Above 95% as determined by SDS-PAGE Analysis.
• Storage: Store at -20 centigrade
• Reconstitution: Centrifuge the vial prior to opening. Rconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein(example 0.1% BSA) and store in working aliquots at -20 centigrade to -80 centigrade.

Gene Information

• Gene Name: GDF15 growth differentiation factor 15 [ Homo sapiens ]
• Official Symbol: GDF15
• Synonyms: GDF15; growth differentiation factor 15; growth/differentiation factor 15; MIC 1; MIC1; NAG 1; PDF; PLAB; prostate differentiation factor; PTGFB; NRG-1; PTGF-beta; placental TGF-beta; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; macrophage inhibitory cytokine 1; placental bone morphogenetic protein; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1; MIC-1; NAG-1; GDF-15
• Gene ID: 9518
• mRNA Refseq: NM_004864
• Protein Refseq: NP_004855
• MIM: 605312
• UniProt ID: Q99988