Recombinant Mouse Tyms Protein, Myc/DDK-tagged

• Cat.No.: Tyms-6756M
• Product Overview: Purified recombinant protein of mouse full-length thymidylate synthase (cDNA clone MGC:28246 IMAGE:3994204), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This gene encodes an enzyme that catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate as a cofactor. This function maintains the thymidine-5-prime monophosphate concentration critical for DNA replication and repair. The encoded enzyme is a target for cancer chemotherapeutic agents. The majority of transcripts for this gene lack a 3' UTR. The stop codon in these transcripts is UAA, compared to the UAG found in the genome and longer transcripts, as the polyA site is located within the stop codon. A related pseudogene has been identified on chromosome 10.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 34.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Tyms thymidylate synthase [ Mus musculus (house mouse) ]
• Official Symbol: Tyms
• Synonyms: Tyms; thymidylate synthase; T; Ts; thymidylate synthase; Tsase; EC 2.1.1.45
• Gene ID: 22171
• mRNA Refseq: NM_021288
• Protein Refseq: NP_067263
• UniProt ID: P07607