Recombinant Human TYMS, His-tagged

• Cat.No.: TYMS-30405TH
• Product Overview: Recombinant fragment, corresponding to amino acids 1-191 of Human Thymidylate Synthase with N terminal His tag; MWt 22kDa.

Specification

• Description: Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase.
• Conjugation: HIS
• Source: E. coli
• Form: Lyophilised:Reconstitute with 97 μl aqua dest.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: MPVAGSELPRRPLPPAAQERDAEPRPPHGELQYLGQIQHI LRCGVRKDDRTGTGTLSVFGMQARYSLRDEFPLLTTKR VFWKGVLEELLWFIKGSTNAKELSSKGVKIWDANGSRD FLDSLGFSTREEGDLGPVYGFQWRHFGAEYRDMESDYS GQGVDQLQRVIDTIKTNPDDRRIIMCAWNPRDLPLMA
• Protein length: 1-191 a.a.

Gene Information

• Gene ID: TYMS thymidylate synthetase [ Homo sapiens ]
• Official Symbol: TYMS
• Synonyms: TYMS; thymidylate synthetase; TS; thymidylate synthase; HsT422; TMS; Tsase