TYMS
-
Official Full Name
thymidylate synthetase -
Overview
Thymidylate synthetase (EC 2.1.1.45) is the enzyme used to generate thymidine monophosphate (dTMP), which is subsequently phosphorylated to thymidine triphosphate for use in DNA synthesis and repair. 5,10-methylenetetrahydrofolate dUMP dihydrofolate dTMP By means of reductive methylation, deoxyuridine monophosphate (dUMP) and N5,N10-methylene tetrahydrofolate are together used to form dTMP, yielding dihydrofolate as a secondary product. This provides the sole de novo pathway for production of dTMP and is the only enzyme in folate metabolism in which the 5,10-methylenetetrahydrofolate is oxidised during one-carbon transfer. The enzyme is essential for regulating the balanced supply of the 4 DNA precursors in normal DNA replication: defects in the enzyme activity affecting the regulation process cause various biological and genetic abnormalities, such as thymineless death.[3] The enzyme is an important target for certain chemotherapeutic drugs. Thymidylate synthase is an enzyme of about 30 to 35 Kd in most species except in protozoan and plants where it exists as a bifunctional enzyme that includes a dihydrofolate reductase domain.[2] A cysteine residue is involved in the catalytic mechanism (it covalently binds the 5,6-dihydro-dUMP intermediate). The sequence around the active site of this enzyme is conserved from phages to vertebrates. As an anti-cancer chemotherapy target, thymidylate synthetase can be inhibited by the thymidylate synthase inhibitors such as fluorinated pyrimidine fluorouracil, or certain folate analogues, the most notable one being raltitrexed (trade name Tomudex). -
Synonyms
d TMP synthase;EC 2.1.1.45;HsT422;MGC88736;OTTHUMP00000162195;Thymidylate synthase;thymidylate synthetase;TMS;TS;TSase;TYMS;TYMS protein;Tyms thymidylate synthetase
Recombinant Proteins
- Human
- Mouse
- Rat
- Zebrafish
- E.coli
- Mammalian Cell
- HEK293
- Insect Cell
- HEK293T
- E. coli
- Mamanlian cells
- GST
- His
- Myc&DDK
- Non
- His&Fc&Avi
- Flag
Involved Pathway
TYMS involved in several pathways and played different roles in them. We selected most pathways TYMS participated on our site, such as Pyrimidine metabolism,One carbon pool by folate,Metabolic pathways, which may be useful for your reference. Also, other proteins which involved in the same pathway with TYMS were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| Metabolic pathways | ALDOCB,POLE3,XYLT1,PTGES3B,GCNT4,UQCRH,DBH,GNPDA1,UQCRFS1,PLCH2 |
| Pyrimidine metabolism | POLE2,POLR2EB,CMPK1,POLR2K,NME1,TXNRD3,ENTPD5,CTPS2,DPYDA.1,TXNRD2 |
| One carbon pool by folate | MTHFD2L,ATIC,SHMT1,ALDH1L2,ST20-MTHFS,FTCD,MTR,MTHFS,MTHFD2,MTHFD1B |
Protein Function
TYMS has several biochemical functions, for example, cofactor binding,drug binding,folic acid binding. Some of the functions are cooperated with other proteins, some of the functions could acted by TYMS itself. We selected most functions TYMS had, and list some proteins which have the same functions with TYMS. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| drug binding | CYP2D6,PDE2A,CHRNB4,CHRNA7,CYP4B1,CHRNB3,P2RX2,ADH1,SRP54,DHODH |
| mRNA binding | RBM38,CELF4,RBM25,DOM3Z,NANOS2,THOC2,CPSF6,RPS26L,CPSF5,RPS5 |
| folic acid binding | MTHFS,FTCD,DMGDH,SLC19A1,GNMT,DAAM2,FOLR1,SLC46A1,FOLR3,DHFR |
| protein homodimerization activity | BAK1,PLEK,WHRN,FLRT3,PDXK,IMPA2,LGALS6,RAG1,TIRAP,CR2 |
| nucleotide binding | DAPK2A,NIM1,POLR2D,CSNK2A2A,FLJ11011L,RBM39B,MYHZ1.2,HNRNPA0L,ATP11A,PABPC1A |
| cofactor binding | WWOX,ASNS,ME3,DDO,PAH,UROS,ACLY,PHYH,BIRC5,PGAM2 |
Interacting Protein
TYMS has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with TYMS here. Most of them are supplied by our site. Hope this information will be useful for your research of TYMS.
VCAM1;SH3GL2;Tuba3a
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References
- Rupasree, Y; Naushad, SM; et al. Epigenetic modulation of RFC1, MHC2TA and HLA-DR in systemic lupus erythematosus: Association with serological markers and six functional polymorphisms of one-carbon metabolic pathway. GENE 536:45-52(2014).
