Recombinant Human TYMS Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: TYMS-1408H
• Product Overview: TYMS MS Standard C13 and N15-labeled recombinant protein (NP_001062) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 35.5 kDa
• AA Sequence: MPVAGSELPRRPLPPAAQERDAEPRPPHGELQYLGQIQHILRCGVRKDDRTGTGTLSVFGMQARYSLRDEFPLLTTKRVFWKGVLEELLWFIKGSTNAKELSSKGVKIWDANGSRDFLDSLGFSTREEGDLGPVYGFQWRHFGAEYRDMESDYSGQGVDQLQRVIDTIKTNPDDRRIIMCAWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFKAEDFQIEGYNPHPTIKMEMAVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: TYMS thymidylate synthetase [ Homo sapiens (human) ]
• Official Symbol: TYMS
• Synonyms: TYMS; thymidylate synthetase; TS; thymidylate synthase; HsT422; TMS; Tsase; TSase; HST422; MGC88736
• Gene ID: 7298
• mRNA Refseq: NM_001071
• Protein Refseq: NP_001062
• MIM: 188350
• UniProt ID: P04818