Recombinant Mouse Spp1 Protein, His-tagged

• Cat.No.: Spp1-2095M
• Product Overview: Recombinant Mouse Spp1 Protein (Val138-Asn294) with N-His tag was expressed in E. coli.

Specification

• Description: Enables extracellular matrix binding activity. Acts upstream of or within several processes, including cellular ion homeostasis; cellular response to leukemia inhibitory factor; and neutrophil chemotaxis. Located in apical part of cell and cytoplasm. Is expressed in several structures, including alimentary system; brain; metanephros; reproductive system; and skeleton. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); biliary atresia; coronary artery disease (multiple); disease of cellular proliferation (multiple); and hepatitis. Orthologous to human SPP1 (secreted phosphoprotein 1).
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 21.2 kDa
  Accurate Molecular Mass: 35 kDa
• Protein length: Val138-Asn294
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Spp1 secreted phosphoprotein 1 [ Mus musculus (house mouse) ]
• Official Symbol: Spp1
• Synonyms: Spp1; secreted phosphoprotein 1; OP; 2AR; Bsp; Eta; Opn; Ric; BNSP; BSPI; Opnl; Apl-1; ETA-1; Spp-1; osteopontin; 44 kDa bone phosphoprotein; bone sialoprotein 1; calcium oxalate crystal growth inhibitor protein; early T-lymphocyte activation 1 protein; minopontin; osteopontin-like protein
• Gene ID: 20750
• mRNA Refseq: NM_009263
• Protein Refseq: NP_033289
• UniProt ID: P10923