Recombinant Human Secreted Phosphoprotein 1, His-tagged

• Cat.No.: SPP1-5129H
• Product Overview: Recombinant human Osteopontin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography

Specification

• Cat. No.: SPP1-5129H
• Description: Osteopontin (OPN), also known as Secreted phosphoprotein 1 (SPP1), is a human gene product which is conserved in other species. This protein is a glycoprotein that was first identified in osteoblasts and plays an important role in bone remodeling, immune functions in fibroblasts, macrophages, and lymphocytes during inflammation and wound healing.
• Concentration: 1 mg/ml
• Source: Human
• Host: E.coli
• Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 1mM DTT, 10% glycerol, and 2mM EDTA.
• Purity: > 90% by SDS - PAGE
• Sequence: 17-314 amino acids: MGSSHHHHHH SSGLVPRGSH RSMIPVKQAD SGSSEEKQLY NKYPDAVATW LNPDPSQKQN LLAPQNAVSS EETNDFKQET LPSKSNESHD HMDDMDDEDD DDHVDSQDSI DSNDSDDVDD TDDSHQSDES HHSDESDELV TDFPTDLPAT EVFTPVVPTV DTYDGRGDSV VYGLRSKSKK FRRPDIQYPD ATDEDITSHM ESEELNGAYK AIP
• Molecular Mass: 36.2 kDa (321aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
• Applications: SDS-PAGE
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: SPP1 secreted phosphoprotein 1 [ Homo sapiens ]
• Official Symbol: SPP1
• Synonyms: SPP1; secreted phosphoprotein 1; OPN; BNSP; BSPI; ETA-1; MGC110940; osteopontin; SPP-1; uropontin; nephropontin; osteopontin-C; osteopontin-D; OTTHUMP00000161173; OTTHUMP00000161174; OTTHUMP00000218555; OTTHUMP00000218556; SPP1/CALPHA1 fusion; bone sialoprotein 1; bone sialoprotein I; urinary stone protein; early T-lymphocyte activation 1; secreted phosphoprotein-1 (osteopontin, bone sialoprotein); osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
• Gene ID: 6696
• mRNA Refseq: NM_000582
• Protein Refseq: NP_000573
• MIM: 166490
• UniProt ID: P10451
• Chromosome Location: 4q22.1
• Pathway: Direct p53 effectors; ECM-receptor interaction; Endochondral Ossification; FGF signaling pathway; Focal Adhesion; Integrin cell surface interactions; Integrins in angiogenesis; Osteoclast Signaling; Osteopontin-mediated events; Signaling by PDGF; TGF Beta Signaling Pathway; Toll-like receptor signaling pathway
• Function: cytokine activity; extracellular matrix binding