Recombinant Mouse Runx2 protein, His-tagged

• Cat.No.: Runx2-5723M
• Product Overview: Recombinant Mouse Runx2 aa. (Ser125~Ala352 (Accession # Q08775)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a member of the runt domain-containing family of transcription factors. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 26.5kDa
• Protein length: Ser125~Ala352
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Runx2 runt related transcription factor 2 [ Mus musculus (house mouse) ]
• Official Symbol: Runx2
• Synonyms: Runx2; runt related transcription factor 2; Cbf; LS3; AML3; Osf2; Cbfa1; Cbfa-1; PEBP2aA; Pebp2a1; Pebpa2a; runt-related transcription factor 2; AKV core binding factor; CBF-alpha-1; PEA2-alpha A; PEBP2 alpha A; SL3-3 enhancer factor 1 alpha A subunit; acute myeloid leukemia 3 protein; core binding factor alpha 1; osteoblast-specific transcription factor 2; polyomavirus enhancer-binding protein 2 alpha A subunit; runt domain, alpha subunit 1
• Gene ID: 12393
• mRNA Refseq: NM_001146038.2
• Protein Refseq: NP_001139510.1
• UniProt ID: Q08775