Recombinant Mouse Ren1 Protein, His-tagged

• Cat.No.: Ren1-379M
• Product Overview: Recombinant mouse REN1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Description: REN1, also known as Renin-1, is a member of the peptidase A1 family. It is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration. Androgen and thyroid hormones influence levels of Renin-1 in mouse submandibular gland (SMG) primarily by regulating the amount of Renin-1 mRNA available for translation. This protein is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Also, REN1 was found to be co localized with the lysosomal marker, beta-glucuronidase, by double-fluorescent labeling.
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 42.5kDa (387aa)40-57KDa (SDS-PAGE under reducing conditions.)
• AA Sequence: LPTRTATFER IPLKKMPSVR EILEERGVDM TRLSAEWGVF TKRPSLTNLT SPVVLTNYLN TQYYGEIGIG TPPQTFKVIF DTGSANLWVP STKCSRLYLA CGIHSLYESS DSSSYMENGS DFTIHYGSGR VKGFLSQDSV TVGGITVTQT FGEVTELPLI PFMLAKFDGV LGMGFPAQAV GGVTPVFDHI LSQGVLKEEV FSVYYNRGSH LLGGEVVLGG SDPQHYQGNF HYVSISKTDS WQITMKGVSV GSSTLLCEEG CAVVVDTGSS FISAPTSSLK LIMQALGAKE KRIEEYVVNC SQVPTLPDIS FDLGGRAYTL SSTDYVLQYP NRRDKLCTLA LHAMDIPPPT GPVWVLGATF IRKFYTEFDR HNNRIGFALA RHHHHHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: Ren1 renin 1 structural [ Mus musculus ]
• Official Symbol: Ren1
• Synonyms: REN1; renin 1 structural; renin-1; renin b; kidney renin; aspartyl-protease; angiotensinogenase; Ren; Rnr; Rn-1; Ren-1; Ren-A; Ren1c; Ren1d; D19352
• Gene ID: 19701
• mRNA Refseq: NM_031192
• Protein Refseq: NP_112469
• Pathway: ACE Inhibitor Pathway, organism-specific biosystem; Renin-angiotensin system, organism-specific biosystem; Renin-angiotensin system, conserved biosystem
• Function: aspartic-type endopeptidase activity; endopeptidase activity; endopeptidase activity; hydrolase activity; insulin-like growth factor receptor binding; peptidase activity; receptor binding