Active Recombinant Mouse Ren1 protein, His-tagged

• Cat.No.: Ren1-2305M
• Product Overview: Recombinant Mouse Ren1 protein(NP_112469.1)(Met1-Arg402), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-402 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Measured by its ability to cleave the fluorogenic peptide substrate 5­FAM/QXL 520 (Peti­Peterdi, J. et al., 2009, Physiology 24:88.). The specific activity is > 20 pmoles/min/μg.2. Immobilized mouse REN1-His at 10ug/ml (100 μl/well) can bind biotinylated human AGT-His with a linear range of 31.25-250 ng/ml.
• Molecular Mass: The recombinant mouse REN1 consists of 392 amino acids and has a predicted molecular mass of 43.2 kDa. As a result of glycosylation, the apparent molecular mass of rmREN1 is approximately 48-55 kDa in SDS-PAGE under reducing conditions.
• Proteinlength: Met1-Arg402
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ren1 renin 1 structural [ Mus musculus ]
• Official Symbol: Ren1
• Synonyms: REN1; renin 1 structural; renin-1; renin b; kidney renin; aspartyl-protease; angiotensinogenase; Ren; Rnr; Rn-1; Ren-1; Ren-A; Ren1c; Ren1d; D19352
• Gene ID: 19701
• mRNA Refseq: NM_031192
• Protein Refseq: NP_112469