Recombinant Mouse Prkra Protein, Myc/DDK-tagged

• Cat.No.: Prkra-5127M
• Product Overview: Purified recombinant protein of mouse full-length protein kinase, interferon inducible double stranded RNA dependent activator (Prkra), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner.
• Molecular Mass: 34.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Prkra protein kinase, interferon inducible double stranded RNA dependent activator [ Mus musculus (house mouse) ]
• Official Symbol: Prkra
• Synonyms: PRKRA; protein kinase, interferon inducible double stranded RNA dependent activator; interferon-inducible double stranded RNA-dependent protein kinase activator A; PKR-associated protein X; PKR-associating protein X; protein activator of the interferon-induced protein kinase; protein kinase, interferon-inducible double stranded RNA-dependent activator; PRK; RAX; Pact; lear; AV120107
• Gene ID: 23992
• mRNA Refseq: NM_011871
• Protein Refseq: NP_036001
• UniProt ID: Q9WTX2