Recombinant Mouse Polr2j Protein, Myc/DDK-tagged

• Cat.No.: Polr2j-5000M
• Product Overview: Purified recombinant protein of mouse full-length polymerase (RNA) II (DNA directed) polypeptide J (Polr2j), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 13.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Polr2j polymerase (RNA) II (DNA directed) polypeptide J [ Mus musculus (house mouse) ]
• Official Symbol: Polr2j
• Synonyms: POLR2J; polymerase (RNA) II (DNA directed) polypeptide J; DNA-directed RNA polymerase II subunit RPB11; RPB14; RNA polymerase II subunit B11; RNA polymerase II subunit RPB14; RNA polymerase II 13.3 kDa subunit; RNA polymerase II 4 (14 kDa subunit); DNA-directed RNA polymerase II subunit J; polymerase (RNA) II (DNA directed) polypeptide I; DNA-directed RNA polymerase II 13.3 kDa polypeptide; Polr2i; Rpb11a; Rpo2-4; 14.5kDa
• Gene ID: 20022
• mRNA Refseq: NM_011293
• Protein Refseq: NP_035423
• UniProt ID: Q3TYI2