Recombinant Human Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa, His-tagged

• Cat.No.: POLR2J-7206H
• Product Overview: Recombinant human POLR2J protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 15.7 kDa.

Specification

• Description: POLR2J is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This protein exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 5mM DTT, and 2mM EDTA.
• Molecular Mass: 15.7 kDa (140aa) confirmed by MALDI-TOF
• Protein length: 1-117aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNAPPAF ESFLLFEGEK KITINKDTKV PNACLFTINK EDHTLGNIIK SQLLKDPQVL FAGYKVPHPL EHKIIIRVQT TPDYSPQEAF TNAITDLISE LSLLEERFRV AIKDKQEGIE
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: POLR2J polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa [ Homo sapiens ]
• Official Symbol: POLR2J
• Synonyms: POLR2J; polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa; polymerase (RNA) II (DNA directed) polypeptide J (13.3kD); DNA-directed RNA polymerase II subunit RPB11-a; hRPB14; POLR2J1; RPB11; RPB11A; RPB11m; RNA polymerase II subunit B11-a; RNA polymerase II 13.3 kDa subunit; DNA-directed RNA polymerase II subunit J-1; MGC71910
• Gene ID: 5439
• mRNA Refseq: NM_006234
• Protein Refseq: NP_006225
• MIM: 604150
• UniProt ID: P52435
• Chromosome Location: 7q11.2
• Pathway: Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; DNA Repair, organism-specific biosystem; Disease, organism-specific biosystem; Dual incision reaction in TC-NER, organism-specific biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem; Formation of HIV-1 elongation complex containing HIV-1 Tat, organism-specific biosystem; Formation of HIV-1 elongation complex in the absence of HIV-1 Tat, organism-specific biosystem
• Function: DNA binding; DNA-directed RNA polymerase activity; LRR domain binding; protein binding; contributes_to protein kinase activity