Recombinant Human POLR2J 293 Cell Lysate

Description :	Antigen standard for polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa (POLR2J) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source :	HEK 293 cells
Species :	Human
Components :	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size :	0.1 mg
Storage Instruction :	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications :	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag :	Non

Gene Name	POLR2J polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa [ Homo sapiens ]
Official Symbol	POLR2J
Synonyms	POLR2J; polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa; polymerase (RNA) II (DNA directed) polypeptide J (13.3kD); DNA-directed RNA polymerase II subunit RPB11-a; hRPB14; POLR2J1; RPB11; RPB11A; RPB11m; RNA polymerase II subunit B11-a; RNA polymerase II 13.3 kDa subunit; DNA-directed RNA polymerase II subunit J-1; MGC71910;
Gene ID	5439
mRNA Refseq	NM_006234
Protein Refseq	NP_006225
MIM	604150
UniProt ID	P52435
Chromosome Location	7q11.2
Pathway	Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; DNA Repair, organism-specific biosystem; Disease, organism-specific biosystem; Dual incision reaction in TC-NER, organism-specific biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem; Formation of HIV-1 elongation complex containing HIV-1 Tat, organism-specific biosystem; Formation of HIV-1 elongation complex in the absence of HIV-1 Tat, organism-specific biosystem;
Function	DNA binding; DNA-directed RNA polymerase activity; LRR domain binding; protein binding; contributes_to protein kinase activity;

COA