Recombinant mouse Pgam1, His-tagged

• Cat.No.: Pgam1-15836M
• Product Overview: Recombinant mouse PGAM1 protein, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Description: PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Recombinant mouse PGAM1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
• Molecular Mass: 31.4 kDa (278aa) confirmed by MALDI-TOF
• Protein length: 1-254aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAAYKL VLIRHGESAW NLENRFSGWY DADLSPAGHE EAKRGGQALR DAGYEFDICF TSVQKRAIRT LWTVLDAIDQ MWLPVVRTWR LNERHYGGLT GLNKAETAAK HGEAQVKIWR RSYDVPPPPM EPDHPFYSNI SKDRRYADLT EDQLPSCESLKDTIARALPF WNEEIVPQIK EGKRVLIAAH GNSLRGIVKH LEGLSEEAIM ELNLPTGIPI VYELDKNLKP IKPMQFLGDE ETVRKAMEAVAAQGKVKK
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term. For long term storage, aliquot and store at at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: Pgam1 phosphoglycerate mutase 1 [ Mus musculus ]
• Official Symbol: PGAM1
• Synonyms: PGAM1; phosphoglycerate mutase 1; PGAM-B; BPG-dependent PGAM 1; phosphoglycerate mutase isozyme B; Pgam-1; 2310050F24Rik; MGC102422; MGC118049
• Gene ID: 18648
• mRNA Refseq: NM_023418
• Protein Refseq: NP_075907
• Pathway: Gluconeogenesis, organism-specific biosystem; Gluconeogenesis, oxaloacetate => fructose-6P, organism-specific biosystem; Gluconeogenesis, oxaloacetate => fructose-6P, conserved biosystem; Glucose metabolism, organism-specific biosystem; Glycine, serine and threonine metabolism, organism-specific biosystem
• Function: phosphoglycerate mutase activity; phosphoglycerate mutase activity; protein kinase binding