Recombinant Mouse Grem2, His-tagged

• Cat.No.: Grem2-7387M
• Product Overview: A DNA sequence encoding the mouse GREM1(Met1-Asp184) was expressed in Baculovirus, with a His tag at the C-terminus.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Protein Length: 1-184 a.a.
• Description: GREM1 is a member of the DAN family. It contains 1 CTCK (C-terminal cystine knot-like) domain and is highly expressed in small intestine, fetal brain and colon. As a cytokine, GREM1 may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. It interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner and down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis. GREM1 also is an inhibitor in the TGF beta signaling pathway. It inhibits BMP-2, -4, and -7. Inhibition by grem 1 of BMPs in mice allow the expression of fibroblast growth factors (FGFs) 4 and 8 and Sonic hedgehog (SHH) which are necessary for proper limb development.
• Predicted N Terminal: Lys 25
• Form: Lyophilized from a 0.2μM filtered solution of 20mM Tris, 500mM NaCl, pH7.4,10% gly,0.5mM EDTA, 3mM DTT. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The recombinant protein consists of 171 amino acids and has a calculated molecular mass of 19.7 kDa, migrates as an approximately 25 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: 1.0 eu/μg of the protein as determined by the lal
• Purity: >97 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Grem2 gremlin 2 homolog, cysteine knot superfamily (Xenopus laevis) [ Mus musculus ]
• Official Symbol: Grem2
• Synonyms: GREM2; gremlin 2 homolog, cysteine knot superfamily (Xenopus laevis); gremlin-2; Gremlin1; protein related to DAN and cerberus; cysteine knot superfamily 1, BMP antagonist 2; Prdc; Gremlin2
• Gene ID: 23893
• mRNA Refseq: NM_011825
• Protein Refseq: NP_035955
• Pathway: Signal Transduction, organism-specific biosystem; Signaling by BMP, organism-specific biosystem
• Function: cytokine activity