Recombinant Mouse Apoc3 protein, His-tagged

• Cat.No.: Apoc3-1939M
• Product Overview: Recombinant Mouse Apoc3 aa. (Glu21~Ser99) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes an apolipoprotein which is the major protein component of very-low-density lipoproteins (VLDL) and a minor component of high-density lipoproteins (HDL). The encoded protein is thought to regulate the metabolism of triglyceride-rich lipoproteins and play a role in lipid storage and the mobilization of fat cells. This gene is clustered with three other apolipoprotein genes on chromosome 9 and is associated with coronary disease. Mice lacking this gene have lower levels of total cholesterol in the plasma. Mutations in the human genes causes hyperalphalipoproteinemia 2, a disorder of lipid metabolism which results in a favorable lipid profile (lower LDL-cholesterol, higher HDL-cholesterol and lower levels of serum triglycerides when fasting and after a meal). Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 21kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Glu21~Ser99
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 92%
• Characteristic: The isoelectric point is 5.1.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage Buffer: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Apoc3 apolipoprotein C-III [ Mus musculus (house mouse) ]
• Official Symbol: Apoc3
• Synonyms: Myo1d; myosin ID; AW544947; D11Ertd9e; myosin-1d; 9930104H07Rik; unconventional myosin-Id; myo1d; myosin-Id
• Gene ID: 11814
• mRNA Refseq: NM_001289755.1
• Protein Refseq: NP_001276684.1
• UniProt ID: E9QP56