Recombinant Human VCL Protein, His-tagged

• Cat.No.: VCL-554H
• Product Overview: Recombinant Human VCL Protein (Val3-Arg485) with N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Val3-Arg485
• Description: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 56.6 kDa
  Accurate Molecular Mass: 57 kDa
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: VCL vinculin [ Homo sapiens (human) ]
• Official Symbol: VCL
• Synonyms: VCL; vinculin; metavinculin; MVCL; CMD1W; CMH15
• Gene ID: 7414
• mRNA Refseq: NM_003373
• Protein Refseq: NP_003364
• MIM: 193065
• UniProt ID: P18206