Recombinant Human VCL protein

• Cat.No.: VCL-230H
• Product Overview: Recombinant Human VCL, transcript variant 2, was expressed in E. coli.

Specification

• Description: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
• Source: E. coli
• Species: Human
• Form: Lyophilized from a 0.2 µM filtered solution of 20mM PB, 150mM NaCl, pH 7.2
• Molecular Mass: 117kD
• AA Sequence: Met1- Gln1066
• Endotoxin: Endotoxin level is <0.1 ng/µg of protein (<1EU/µg).
• Purity: >95% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Tag: Non

Gene Information

• Gene Name: VCL vinculin [ Homo sapiens ]
• Official Symbol: VCL
• Synonyms: VCL; vinculin; metavinculin; MVCL; CMD1W; CMH15
• Gene ID: 7414
• mRNA Refseq: NM_003373
• Protein Refseq: NP_003364
• MIM: 193065
• UniProt ID: P18206