Recombinant Human UNG Full Length protein, His-tagged

• Cat.No.: UNG-3601H
• Product Overview: Recombinant Human UNG(Met1~Leu313) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene (GeneID 10309), which has been confused with this gene, in the literature and some databases.
• Source: E. coli
• Species: Human
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300
• Molecular Mass: 38.3kDa
• Protein length: Met1~Leu313
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: UNG uracil-DNA glycosylase [ Homo sapiens ]
• Official Symbol: UNG
• Synonyms: UNG; uracil-DNA glycosylase; DGU; HIGM4; UDG; UNG1; UNG2; uracil DNA glycosylase 1; uracil DNA glycosylase 2; uracil-DNA glycosylase 1, uracil-DNA glycosylase 2; UNG15; DKFZp781L1143
• Gene ID: 7374
• mRNA Refseq: NM_003362
• Protein Refseq: NP_003353
• MIM: 191525
• UniProt ID: P13051
• Chromosome Location: 12q23-q24.1
• Pathway: Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem; Primary immunodeficiency, organism-specific biosystem; Primary immunodeficiency, conserved biosystem
• Function: hydrolase activity, hydrolyzing N-glycosyl compounds; uracil DNA N-glycosylase activity; uracil DNA N-glycosylase activity

Reviews

09/22/2022

The technician guided me through the process, which led to satisfactory results.

07/14/2022

The UNG protein product received was as advertised, top quality and good value for money.

05/04/2022

Prompt delivery helped me get satisfactory experimental results in a short period of time.

Q&As

What biological processes are UNG involved in?

UNG is involved in DNA repair, base excision repair and apoptosis.

Where is UNG located?

UNG is localized in the nucleus, nucleoplasm, and mitochondria.

What protein does UNG shear to produce?

UNG can be sheared to produce UNG1 and UNG2.

Where is UNG primarily expressed?

UNG is mainly expressed in testis, thymus and skeletal muscle.