Recombinant Human UBE2D3 Protein, His-tagged

• Cat.No.: UBE2D3-2004H
• Product Overview: Recombinant Human UBE2D3 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Met147) of human UBE2D3 (Accession #NP_003331.1) fused with a 6×His tag at the C-terminus.

Specification

• Description: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5 % mannitol.
• Protein length: Met1-Met147
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90% as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: UBE2D3 ubiquitin conjugating enzyme E2 D3 [ Homo sapiens (human) ]
• Official Symbol: UBE2D3
• Synonyms: E2(17)KB3;UBC4/5;UBCH5C
• Gene ID: 7323
• mRNA Refseq: NM_181893
• Protein Refseq: NP_003331.1
• MIM: 602963
• UniProt ID: P61077