Recombinant Human TYMS, His-tagged

• Cat.No.: TYMS-30404TH
• Product Overview: Recombinant human Thymidylate synthase protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-313aa
• Description: Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT.
• Molecular Mass: 37.8 kDa (333aa), confirmed by MALDI-TOF.
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MPVAGSELPR RPLPPAAQER DAEPRPPHGE LQYLGQIQHI LRCGVRKDDR TGTGTLSVFG MQARYSLRDEFPLLTTKRVF WKGVLEELLW FIKGSTNAKE LSSKGVKIWD ANGSRDFLDS LGFSTREEGD LGPVYGFQWR HFGAEYRDME SDYSGQGVDQLQRVIDTIKT NPDDRRIIMC AWNPRDLPLM ALPPCHALCQ FYVVNSELSC QLYQRSGDMG LGVPFNIASY ALLTYMIAHI TGLKPGDFIHTLGDAHIYLN HIEPLKIQLQ REPRPFPKLR ILRKVEKIDD FKAEDFQIEG YNPHPTIKME MAV
• Purity: > 95% by SDS - PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TYMS thymidylate synthetase [ Homo sapiens (human) ]
• Official Symbol: TYMS
• Synonyms: TYMS; TS; HsT422; MGC88736; TMS; TSase; thymidylate synthetase; HST422; MGC88736; EC 2.1.1.45; Tsase.
• Gene ID: 7298
• mRNA Refseq: NM_001071
• Protein Refseq: NP_001062
• MIM: 188350
• UniProt ID: P04818
• Chromosome Location: 18p11.32
• Pathway: Metabolic pathways; One carbon pool by folate; Pyrimidine metabolism; Cell Cycle, Mitotic; Metabolism of nucleotides.
• Function: cofactor binding; methyltransferase activity; nucleotide binding; thymidylate synthase activity; transferase activity.