Recombinant Human TYMS protein, MYC/DDK-tagged

• Cat.No.: TYMS-684H
• Product Overview: Recombinant Human TYMS fused with MYC/DDK tag at C-terminal was expressed in HEK293.

Specification

• Description: Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Form: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 35.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: TYMS thymidylate synthetase [ Homo sapiens ]
• Official Symbol: TYMS
• Synonyms: TYMS; thymidylate synthetase; TS; thymidylate synthase; HsT422; TMS; Tsase; TSase; HST422; MGC88736
• Gene ID: 7298
• mRNA Refseq: NM_001071
• Protein Refseq: NP_001062
• MIM: 188350
• UniProt ID: P04818
• Chromosome Location: 18p11.31-p11.21
• Pathway: Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; E2F mediated regulation of DNA replication, organism-specific biosystem; E2F transcription factor network, organism-specific biosystem; Fluoropyrimidine Activity, organism-specific biosystem; G1/S Transition, organism-specific biosystem; G1/S-Specific Transcription, organism-specific biosystem
• Function: cofactor binding; folic acid binding; methyltransferase activity; nucleotide binding; thymidylate synthase activity; thymidylate synthase activity; transferase activity