Recombinant Human Troponin C Type 1 (slow), His-tagged

• Cat.No.: TNNC1-6896H
• Product Overview: Recombinant human TNNC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 20.5 kDa.

Specification

• Description: TNNC1 is a member of troponins family. Troponin has 3 subunits, Troponin I(Tn-I), Troponin T(Tn-T) and Troponin C(Tn-C). Tn-I subunit inhibits actomyosin ATPase and Tn-T subunit binds tropomyosin and Tn-C, while Tn-C subunit binds calcium and overcomes the inhibitory action of the troponin complex on actin filaments. TNNC1 is dumbbell-shaped and has a hydrophobic pocket that increases the contractile force of muscle fibers.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl.
• Molecular Mass: 20.5 kDa (181aa), confirmed by MALDI-TOF
• Protein length: 1-161aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGCISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRCMKDDSK GKSEEELSDL FRMFDKNADG YIDLDELKIM LQATGETITE DDIEELMKDG DKNNDGRIDY DEFLEFMKGV E
• Purity: >95% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20℃ or -70℃. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TNNC1 troponin C type 1 (slow) [ Homo sapiens ]
• Official Symbol: TNNC1
• Synonyms: TNNC1; troponin C type 1 (slow); TNNC, troponin C, slow; troponin C, slow skeletal and cardiac muscles; troponin C1, slow; cardiac troponin C; slow twitch skeletal/cardiac muscle troponin C; TNC; TN-C; TNNC; CMD1Z; CMH13
• Gene ID: 7134
• mRNA Refseq: NM_003280
• Protein Refseq: NP_003271
• MIM: 191040
• UniProt ID: P63316
• Chromosome Location: 3p21.1
• Pathway: Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Cardiac muscle contraction, organism-specific biosystem; Cardiac muscle contraction, conserved biosystem; Dilated cardiomyopathy, organism-specific biosystem; Dilated cardiomyopathy, conserved biosystem; Hypertrophic cardiomyopathy (HCM), organism-specific biosystem
• Function: actin filament binding; calcium ion binding; calcium-dependent protein binding; protein binding; protein homodimerization activity; troponin I binding; troponin I binding; troponin T binding