Recombinant Human TRIM21, His-tagged

• Cat.No.: TRIM21-115H
• Product Overview: TRIM21 is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.

Specification

• Description: This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.
• Source: Sf9 insect cells.
• Species: Human
• Tag: His
• Form: Sterile Filtered clear solution. TRIM21 is supplied in 20mM HEPES pH-7.5, 0.01mM EDTA and 0.02% SDS.
• Molecular Mass: 54,922 Dalton
• Purity: Greater than 90% as determined by SDS-PAGE.
• Applications: Western blot with: a) SLE sera (secondary Sjogren’s syndrome). b) Monoclonal anti-hexa-His-tag antibody.
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Gene Information

• Gene Name: TRIM21 tripartite motif containing 21 [ Homo sapiens (human) ]
• Official Symbol: TRIM21
• Synonyms: TRIM21; SSA; RO52; SSA1; RNF81; tripartite motif containing 21; E3 ubiquitin-protein ligase TRIM21; SS-A; ro(SS-A); 52 kDa Ro protein; RING finger protein 81; Sicca syndrome antigen A; tripartite motif-containing 21; sjoegren syndrome type A antigen; tripartite motif-containing protein 21; 52 kDa ribonucleoprotein autoantigen Ro/SS-A; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro)
• Gene ID: 6737
• mRNA Refseq: NM_003141
• Protein Refseq: NP_003132
• MIM: 109092
• UniProt ID: P19474
• Chromosome Location: 11p15.5
• Pathway: Adaptive Immune System; Antigen processing: Ubiquitination & Proteasome degradation; Cytosolic sensors of pathogen-associated DNA
• Function: DNA binding; RNA binding; ubiquitin-protein ligase activity