Recombinant Human TLE1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: TLE1-5906H
• Product Overview: TLE1 MS Standard C13 and N15-labeled recombinant protein (NP_005068) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 83.2 kDa
• AA Sequence: MFPQSRHPTPHQAAGQPFKFTIPESLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNTICAQVIPFLSQEHQQQVAQAVERAKQVTMAELNAIIGQQQLQAQHLSHGHGPPVPLTPHPSGLQPPGIPPLGGSAGLLALSSALSGQSHLAIKDDKKHHDAEHHRDREPGTSNSLLVPDSLRGTDKRRNGPEFSNDIKKRKVDDKDSSHYDSDGDKSDDNLVVDVSNEDPSSPRASPAHSPRENGIDKNRLLKKDASSSPASTASSASSTSLKSKEMSLHEKASTPVLKSSTPTPRSDMPTPGTSATPGLRPGLGKPPAIDPLVNQAAAGLRTPLAVPGPYPAPFGMVPHAGMNGELTSPGAAYASLHNMSPQMSAAAAAAAVVAYGRSPMVGFDPPPHMRVPTIPPNLAGIPGGKPAYSFHVTADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAVTISNPTRHVYTGGKGCVKVWDISHPGNKSPVSQLDCLNRDNYIRSCKLLPDGCTLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVNKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISVDDKYIVTGSGDKKATVYEVIYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: TLE1 TLE family member 1, transcriptional corepressor [ Homo sapiens (human) ]
• Official Symbol: TLE1
• Synonyms: TLE1; transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila); transducin like enhancer of split 1, homolog of Drosophila E(sp1); transducin-like enhancer protein 1; enhancer of split groucho 1; ESG; ESG1; GRG1; e(Sp1) homolog; enhancer of split groucho-like protein 1; transducin-like enhancer of split 1, homolog of Drosophila E(sp1)
• Gene ID: 7088
• mRNA Refseq: NM_005077
• Protein Refseq: NP_005068
• MIM: 600189
• UniProt ID: Q04724