Recombinant Human TLE1 293 Cell Lysate

• Cat.No.: TLE1-1051HCL

Specification

• Description: Antigen standard for transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: TLE1 transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) [ Homo sapiens ]
• Official Symbol: TLE1
• Synonyms: TLE1; transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila); transducin like enhancer of split 1, homolog of Drosophila E(sp1); transducin-like enhancer protein 1; enhancer of split groucho 1; ESG; ESG1; GRG1; e(Sp1) homolog; enhancer of split groucho-like protein 1; transducin-like enhancer of split 1, homolog of Drosophila E(sp1)
• Gene ID: 7088
• mRNA Refseq: NM_005077
• Protein Refseq: NP_005068
• MIM: 600189
• UniProt ID: Q04724
• Chromosome Location: 9q21.32
• Pathway: Delta-Notch Signaling Pathway, organism-specific biosystem; NOTCH1 Intracellular Domain Regulates Transcription, organism-specific biosystem; Notch-mediated HES/HEY network, organism-specific biosystem; Presenilin action in Notch and Wnt signaling, organism-specific biosystem; Regulation of Wnt-mediated beta catenin signaling and target gene transcription, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by NOTCH, organism-specific biosystem