Recombinant Human SHMT2 Protein (S29-H504), His tagged

• Cat.No.: SHMT2-0225H
• Product Overview: Recombinant Human 6His-TEV-SHMT2(S29-H504 end) Protein was expressed in E. coli.

Specification

• Description: Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis. Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA. Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs. Associates with mitochondrial DNA. In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex: required for IFNAR1 deubiquitination by the BRISC complex.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid
• Protein length: S29-H504
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: SHMT2 serine hydroxymethyltransferase 2 (mitochondrial) [ Homo sapiens (human) ]
• Official Symbol: SHMT2
• Synonyms: SHMT2; serine hydroxymethyltransferase 2 (mitochondrial); SHMT; serine hydroxymethyltransferase, mitochondrial; GLY A+; serine aldolase; serine methylase; threonine aldolase; serine hydroxymethylase; glycine hydroxymethyltransferase; glycine auxotroph A, human complement for hamster; GLYA
• Gene ID: 6472
• mRNA Refseq: NM_001166356
• Protein Refseq: NP_001159828
• MIM: 138450
• UniProt ID: P34897