Recombinant Human SHMT2, His-tagged

• Cat.No.: SHMT2-31356TH
• Product Overview: Recombinant fragment, corresponding to amino acids 376-504 of Human SHMT2 with an N terminal His tag; Predicted MWt 15 kDa.

Specification

• Description: This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants.
• Conjugation: HIS
• Source: E. coli
• Form: Lyophilised:Reconstitute with 125 μl aqua dest.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: GTDNHLVLVDLRPKGLDGARAERVLELVSITANKNTCPGD RSAITPGGLRLGAPALTSRQFREDDFRRVVDFIDEGVN IGLEVKSKTAKLQDFKSFLLKDSETSQRLANLRQRVEQ FARAFPMPGFDEH
• Protein length: 376-504

Gene Information

• Gene Name: SHMT2 serine hydroxymethyltransferase 2 (mitochondrial) [ Homo sapiens ]
• Official Symbol: SHMT2
• Synonyms: SHMT2; serine hydroxymethyltransferase 2 (mitochondrial); SHMT; serine hydroxymethyltransferase, mitochondrial
• Gene ID: 6472
• mRNA Refseq: NM_001166356
• Protein Refseq: NP_001159828
• MIM: 138450
• Uniprot ID: P34897
• Chromosome Location: 12q12-q14
• Pathway: C1-unit interconversion, eukaryotes, organism-specific biosystem; C1-unit interconversion, eukaryotes, conserved biosystem; Cyanoamino acid metabolism, organism-specific biosystem; Cyanoamino acid metabolism, conserved biosystem; Glycine, serine and threonine metabolism, organism-specific biosystem
• Function: L-allo-threonine aldolase activity; amino acid binding; chromatin binding; glycine hydroxymethyltransferase activity; glycine hydroxymethyltransferase activity