Recombinant Human SEMA4A Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: SEMA4A-1573H
• Product Overview: SEMA4A MS Standard C13 and N15-labeled recombinant protein (NP_071762) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is an activator of T-cell-mediated immunity and suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 83.6 kDa
• AA Sequence: MALPALGLDPWSLLGLFLFQLLQLL LPTTTAGGGGQGPMPRVRYYAGDER RALSFFHQKGLQDFDTLLLSGDGNT LYVGAREAILALDIQDPGVPRLKNM IPWPASDRKKSECAFKKKSNETQCF NFIRVLVSYNVTHLYTCGTFAFSPA CTFIELQDSYLLPISEDKVMEGKGQ SPFDPAHKHTAVLVDGMLYSGTMNN FLGSEPILMRTLGSQPVLKTDNFLR WLHHDASFVAAIPSTQVVYFFFEET ASEFDFFERLHTSRVARVCKNDVGG EKLLQKKWTTFLKAQLLCTQPGQLP FNVIRHAVLLPADSPTAPHIYAVFT SQWQVGGTRSSAVCAFSLLDIERVF KGKYKELNKETSRWTTYRGPETNPR PGSCSVGPSSDKALTFMKDHFLMDE QVVGTPLLVKSGVEYTRLAVETAQG LDGHSHLVMYLGTTTGSLHKAVVSG DSSAHLVEEIQLFPDPEPVRNLQLA PTQGAVFVGFSGGVWRVPRANCSVY ESCVDCVLARDPHCAWDPESRTCCL LSAPNLNSWKQDMERGNPEWACASG PMSRSLRPQSRPQIIKEVLAVPNSI LELPCPHLSALASYYWSHGPAAVPE ASSTVYNGSLLLIVQDGVGGLYQCW ATENGFSYPVISYWVDSQDQTLALD PELAGIPREHVKVPLTRVSGGAALA AQQSYWPHFVTVTVLFALVLSGALI ILVASPLRALRARGKVQGCETLRPG EKAPLSREQHLQSPKECRTSASDVD ADNNCLGTEVATRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: SEMA4A semaphorin 4A [ Homo sapiens (human) ]
• Official Symbol: SEMA4A
• Synonyms: SEMA4A; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A; SEMAB; semaphorin-4A; FLJ12287; SemB; sema B; semaphorin-B; RP35; SEMB; CORD10; RP11-54H19.2
• Gene ID: 64218
• mRNA Refseq: NM_022367
• Protein Refseq: NP_071762
• MIM: 607292
• UniProt ID: Q9H3S1

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Q&As

What is the impact of altered SEMA4A activity on neurodegenerative and autoimmune diseases?

Altered SEMA4A activity is associated with the progression of neurodegenerative and autoimmune conditions.

What potential therapeutic applications arise from targeting SEMA4A in neurological and immune disorders?

Targeting SEMA4A offers potential for treating a range of neurological and immune-mediated disorders.

What role does SEMA4A play in immune regulation?

SEMA4A is involved in immune system regulation, affecting immune cell activation and responses.

What is the primary role of SEMA4A in nervous system development?

SEMA4A, a semaphorin, is crucial for guiding axon development and neural connectivity in the nervous system.

How does SEMA4A contribute to axon guidance?

It plays a key role in directing axonal growth and neuronal network formation.

How does SEMA4A interact with other signaling molecules?

SEMA4A works with various signaling pathways, coordinating developmental and immune processes.

How do genetic variations in SEMA4A affect neural and immune functions?

Genetic changes in SEMA4A can lead to impairments in neural development and immune system functioning.