Recombinant Human SEMA4A protein(Met1-His683), hFc-tagged

• Cat.No.: SEMA4A-3925H
• Product Overview: Recombinant Human SEMA4A (NP_071762.2)(Met 1-His 683) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Protein length: Met1-His683
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to bind mouse SEMA4D in a functional ELISA.
• Molecular Mass: The secreted recombinant human SEMA4A/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 893 amino acids and predictes a molecular mass of 99 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh SEMA4A/Fc monomer is approximately 110 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SEMA4A sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A [ Homo sapiens ]
• Official Symbol: SEMA4A
• Synonyms: SEMA4A; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A; SEMAB; semaphorin-4A; FLJ12287; SemB; sema B; semaphorin-B; RP35; SEMB; CORD10; RP11-54H19.2
• Gene ID: 64218
• mRNA Refseq: NM_001193300
• Protein Refseq: NP_001180229
• MIM: 607292
• UniProt ID: Q9H3S1

Reviews

10/29/2022

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05/22/2022

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10/12/2019

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Q&As

What is the impact of altered SEMA4A activity on neurodegenerative and autoimmune diseases?

Altered SEMA4A activity is associated with the progression of neurodegenerative and autoimmune conditions.

What potential therapeutic applications arise from targeting SEMA4A in neurological and immune disorders?

Targeting SEMA4A offers potential for treating a range of neurological and immune-mediated disorders.

What role does SEMA4A play in immune regulation?

SEMA4A is involved in immune system regulation, affecting immune cell activation and responses.

What is the primary role of SEMA4A in nervous system development?

SEMA4A, a semaphorin, is crucial for guiding axon development and neural connectivity in the nervous system.

How does SEMA4A contribute to axon guidance?

It plays a key role in directing axonal growth and neuronal network formation.

How does SEMA4A interact with other signaling molecules?

SEMA4A works with various signaling pathways, coordinating developmental and immune processes.

How do genetic variations in SEMA4A affect neural and immune functions?

Genetic changes in SEMA4A can lead to impairments in neural development and immune system functioning.